Genome Engineering injection (CRISPR injection) Service
(Please read below, service terms included)
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Service Type
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Price (CNY)
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Description
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1680
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Genome Engineering Basic - CRISPR injection only (ship larvae only) | |
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2000
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Genome Engineering Standard - Plan RG plus generation of w+ or y+ transformant (ship transformant). The Cas9-containing chromosome may NOT be removed | |
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2210
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Genome Engineering Premium - Plan RH, with visible marker, plus balancing (ship balanced line). The Cas9-containing chromosome WILL BE removed | |
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2310
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Plan RI plus an early/partial shipment of Plan RH. i.e. ship Plan RH first (this shipment may not contain all lines), then regular Plan RI shipment when finish |
Plan RG - Genome Engineering Basic Service (CNY1680): Similar to the described Plan A. This is an injection-only service and the cost is based on injecting 200-300 embryos. Due to the nature of the Cas9 lines and/or injection mixes, we may inject 250-300 embryos in general. Please send us your CRISPR injection mix (DNAs, RNAs, Oligos, etc) or give us a very clear instruction on how to make the injection mix. You will pay full amount regardless of the number of transformants by NHEJ or HRD found in your lab.
If you prefer to inject to your own strain, please refer to the below "one-time new strain charge" - ￥740 per new strain.
There will be no free second injection in many situations including shipping mistake by the couriers.
Plan RH - Genome Engineering Standard Service (CNY2000): Similar to the described Plan B and the injection procedures for Plan RG. This service includes injection, generation and identification of CRISPR HDR transformants with visible marker (w+ or y+ included as priced; other markers refer to "others and promotions" price table). We will inject your CRISPR mix into 200-300 embryos, back-cross SELECTED survival G0 adults to yw (or suitable stock), expand G1 transformants by crossing to yw (or suitable stock) again, and ship only the G2 CRISPR HDR transformants to you. The Plan RH service does NOT include the removal of the Cas9-containing chromosome.
When using this service, we will try to get at least 1 independent CRISPR HDR transformant, regardless of the size of the gRNA and donor DNA, due to the high variability of the CRISPR designs. We may re-inject (the second injection) for free if there is no transformant from the first injection round; we will not offer further free injection after the second trial.
We will send you maximum 5 lines if there are more found in the screening process.If the injection may contain a fluorescence selection marker, please refer to " Screening for adult eye Fluorescence phenotypes" - ￥370 per service quantity.
Plan RI - Genome Engineering Premium Service (CNY2210): Similar to the described Plan C. This service includes injection, generation, identification of transformants (w+ or y+ included as priced; other markers refer to price table) and balancing. We will balance the CRISPR HDR transformants in addition to the Plan RH service. We may directly setup the G0 crosses using balancers in some situations. The Plan RI service also includes the removal of the Cas9-containing chromosome.
The term of "guarantee" is as of Plan RH and we will send you maximum 5 balanced CRISPR HDR transformants if there are more found in the screening process.
We will take the same measures and charge in the same way for the special situations (i.e. toxic or failure transformation) as described in Plan RH.
Plan RH+RI Service (CNY2310): Plan RI plus an early/partial shipment of Plan RH. i.e. ship Plan RH first (this shipment may not contain all lines), then regular Plan RI shipment when finish.
