P-element transformation services

(Please read below, service terms included)

Plan A - P-element Basic Service (CNY1550): This is an injection-only service and the cost is based on injecting 200 embryos. We obtain DNA sample of the P-element in water from you, then further mix it with the helper plasmid (supplied by us or you) and inject them together into w¹¹¹⁸ or yw embryos. The injected larvae will be shipped to you and you are responsible for identifying your own transformants and balancing them. You will pay full amount regardless of the number of transformants found in your lab.

There will be no free second injection in many situations including shipping mistake by the couriers.

Plan B - P-element Standard Service (CNY1880): This service includes injection, generation and identification of transformants (w⁺ or y⁺ included as priced; other markers refer to price table). We will inject your DNA sample into 200 embryos, back-cross the survival G0 adults to w¹¹¹⁸ or yw, expand G1 transformants by crossing to w¹¹¹⁸ or yw again, and ship only the G2 transformants to you. The G2 transformants will be mailed to you either as adult flies or as larvae.

Injected G0 adults are individually crossed in single vials!
When using this service, we will try to get at least 5 independent transformants for a reasonably sized construct (smaller than 13 kb).

In some special situation, if your injected DNA is lethal, we will inform you at once and perform the injection again (free of charge). If the 2nd injection is lethal again, the charge for the "confirmed" lethal injections (200x2 embryo-injections) is ￥775.

If your injected DNA has no cloning error, is reasonably sized (smaller than 13 kb) and not lethal but we get no transformant, we will perform the injection again (free of charge) and discuss with you the possible reason of this situation and try to fix the problem. we will not offer further free injection after the second trial.

If your injected DNA is larger than 13 kb and we get less than 5 transformants, we will not offer free injection. You are suggested to purchase more service "qty" because usually large constructs are difficult to transform.

Plan C - P-element Premium Service (CNY2650): This is equivalent to "Plan B plus balancing". This service includes injection, identification of transformants and balancing. We will inject your DNA, pick transformants and balance them with balancer chromosomes. We use single balancers. This service takes longer time and you will be charged the full amount regardless of which chromosome(s) the P-elements are incorporated in.

Similar to the description of Plan B above, we will try to get at least 4 independent transformants for a reasonable sized construct (smaller than 13 kb).

We will take the same measure and charge in the same way for the special situations (i.e. failure transformation) as described in Plan B.

Plan B+C - Service (CNY2770): Plan C plus an early/partial shipment of Plan B. i.e. ship Plan B first (this shipment may not contain all lines), then regular Plan C shipment when finish.